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<h1 class="ltx_title ltx_title_section">Merging of reappearing molecules</h1>

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<p class="ltx_p">In some SMLM experiments, a single photoactivated molecule may appear
in several sequential images, then disappear for a while, appear again,
and finally bleach completely. If this is the case, ThunderSTORM identifies
such sequences of molecular locations and combines them into one single
molecule. The new position, imaged size, and the background offset
of the resulting signal is calculated as the mean value of the original
data, while the intensity and the <a href="../estimators/ui/LocalizationUncertainty.html" title="" class="ltx_ref">background signal level</a>
is calculated as the sum. The localization uncertainty of the merged
molecule is calculated from the new values. The distance within which
molecules are merged together in the subsequent frames, as well as
the allowed number of frames in which the molecule can disappear,
are user-specified values. Users can also specify a maximum number
of consecutive frames such that a repeating event is still considered
a single molecule. The merging algorithm is based on finding nearest-neighbors
between active molecules from the previous frame and the current frame.</p>
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<h2 class="ltx_title ltx_title_subsection">See also</h2>

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<span class="ltx_tag ltx_tag_itemize">•</span> 
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<p class="ltx_p"><a href="Postprocessing.html" title="" class="ltx_ref">Post-processing analysis</a></p>
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